The Protective Effect of Eupatilin against Hydrogen Peroxide-Induced Injury Involving 5-Lipoxygenase in Feline Esophageal Epithelial Cells
The Protective Effect of Eupatilin against Hydrogen Peroxide-Induced Injury Involving 5-Lipoxygenase in Feline Esophageal Epithelial Cells
- 대한생리학회-대한약리학회
- The Korean Journal of Physiology & Pharmacology
- 제16권 제5호
- : SCOPUS, SCIE, KCI등재
- 2012.01
- 313 - 320 (8 pages)
In this study, we focused to identify whether eupatilin (5,7-dihydroxy-3 ,4 ,6-trimethoxyflavone), an extract from Artemisia argyi folium, prevents H<sub>2</sub>O<sub>2</sub>-induced injury of cultured feline esophageal epithelial cells. Cell viability was measured by the conventional MTT reduction assay. Western blot analysis was performed to investigate the expression of 5-lipoxygenase by H<sub>2</sub>O<sub>2</sub> treatment in the absence and presence of inhibitors. When cells were exposed to 600 ՌM H<sub>2</sub>O<sub>2</sub> for 24 hours, cell viability was decreased to 40%. However, when cells were pretreated with 25∼150 ՌM eupatilin for 12 hours, viability was significantly restored in a concentration-dependent manner. H<sub>2</sub>O<sub>2</sub>-treated cells were shown to express 5-lipoxygenase, whereas the cells pretreated with eupatilin exhibited reduction in the expression of 5-lipoxygenase. The H<sub>2</sub>O<sub>2</sub>-induced increase of 5-lipoxygenase expression was prevented by SB202190, SP600125, or NAC. We further demonstrated that the level of leukotriene B<sub>4</sub> (LTB<sub>4</sub>) was also reduced by eupatilin, SB202190, SP600125, NAC, or nordihydroguaiaretic acid (a lipoxygenase inhibitor) pretreatment. H<sub>2</sub>O<sub>2</sub> induced the activation of p38MAPK and JNK, this activation was inhibited by eupatilin. These results indicate that eupatilin may reduce H<sub>2</sub>O<sub>2</sub>-induced cytotoxicity, and 5-lipoxygenase expression and LTB<sub>4</sub> production by controlling the p38 MAPK and JNK signaling pathways through antioxidative action in feline esophageal epithelial cells.