상세검색
최근 검색어 전체 삭제
다국어입력
즐겨찾기0
커버이미지 없음
SCOPUS 학술저널

The Protective Effect of Eupatilin against Hydrogen Peroxide-Induced Injury Involving 5-Lipoxygenase in Feline Esophageal Epithelial Cells

The Protective Effect of Eupatilin against Hydrogen Peroxide-Induced Injury Involving 5-Lipoxygenase in Feline Esophageal Epithelial Cells

In this study, we focused to identify whether eupatilin (5,7-dihydroxy-3 ,4 ,6-trimethoxyflavone), an extract from Artemisia argyi folium, prevents H<sub>2</sub>O<sub>2</sub>-induced injury of cultured feline esophageal epithelial cells. Cell viability was measured by the conventional MTT reduction assay. Western blot analysis was performed to investigate the expression of 5-lipoxygenase by H<sub>2</sub>O<sub>2</sub> treatment in the absence and presence of inhibitors. When cells were exposed to 600 &#1356;M H<sub>2</sub>O<sub>2</sub> for 24 hours, cell viability was decreased to 40%. However, when cells were pretreated with 25∼150 &#1356;M eupatilin for 12 hours, viability was significantly restored in a concentration-dependent manner. H<sub>2</sub>O<sub>2</sub>-treated cells were shown to express 5-lipoxygenase, whereas the cells pretreated with eupatilin exhibited reduction in the expression of 5-lipoxygenase. The H<sub>2</sub>O<sub>2</sub>-induced increase of 5-lipoxygenase expression was prevented by SB202190, SP600125, or NAC. We further demonstrated that the level of leukotriene B<sub>4</sub> (LTB<sub>4</sub>) was also reduced by eupatilin, SB202190, SP600125, NAC, or nordihydroguaiaretic acid (a lipoxygenase inhibitor) pretreatment. H<sub>2</sub>O<sub>2</sub> induced the activation of p38MAPK and JNK, this activation was inhibited by eupatilin. These results indicate that eupatilin may reduce H<sub>2</sub>O<sub>2</sub>-induced cytotoxicity, and 5-lipoxygenase expression and LTB<sub>4</sub> production by controlling the p38 MAPK and JNK signaling pathways through antioxidative action in feline esophageal epithelial cells.

로딩중