Effects of CoCl<sub>2</sub> on multi-lineage differentiation of C3H/10T1/2 mesenchymal stem cells
Effects of CoCl<sub>2</sub> on multi-lineage differentiation of C3H/10T1/2 mesenchymal stem cells
- Hong Il Yoo Yeon Hee Moon Min Seok Kim
- 대한생리학회-대한약리학회
- The Korean Journal of Physiology & Pharmacology
- 제20권 제1호
- 등재여부 : KCI등재
- 2016.01
- 53 - 62 (10 pages)
Mesenchymal stem cells (MSCs) in the bone marrow and other somatic tissues reside in an environment with relative low oxygen tension. Cobalt chloride (CoCl<sub>2</sub>) can mimic hypoxic conditions through transcriptional changes of some genes including hypoxia-inducible factor-1a (HIF-1a) and vascular endothelial growth factor (VEGF). This study evaluated the potential role of CoCl<sub>2</sub> preconditioning on multi-lineage differentiation of C3H/10T1/2, a murine MSC line to understand its possible molecular mechanisms <i>in vitro</i>. CoCl<sub>2</sub> treatment of MSCs markedly increased HIF-1a and VEGF mRNA, and protein expression of HIF-1a. Temporary preconditioning of MSCs with CoCl<sub>2</sub> induced up-regulation of osteogenic markers including alkaline phosphatase, osteocalcin, and type I collagen during osteogenic differentiation, followed by enhanced mineralization. CoCl<sub>2</sub> also increased chondrogenic markers including aggrecan, sox9, and type II collagen, and promoted chondrocyte differentiation. CoCl<sub>2</sub> suppressed the expression of adipogenic markers including PPARγ, aP2, and C/EBPa, and inhibited adipogenesis. Temporary preconditioning with CoCl<sub>2</sub> could affect the multi-lineage differentiation of MSCs.