Mitochondrial calcium uniporter inhibition attenuates mouse bone marrow-derived mast cell degranulation induced by beta-1,3-glucan
Mitochondrial calcium uniporter inhibition attenuates mouse bone marrow-derived mast cell degranulation induced by beta-1,3-glucan
- Dang Van Cuong Hyoung Kyu Kim Jubert Marquez Nari Kim Kyung Soo Ko Byoung Doo Rhee Jin Han
- 대한생리학회-대한약리학회
- The Korean Journal of Physiology & Pharmacology
- 제20권 제2호
- 등재여부 : KCI등재
- 2016.01
- 213 - 220 (8 pages)
Mast cells are primary mediators of allergic inflammation. Beta-1,3- glucan (BG) protects against infection and shock by activating immune cells. Activation of the BG receptor induces an increase in intracellular Ca<sup>2+</sup>, which may induce exocytosis. However, little is known about the precise mechanisms underlying BG activation of immune cells and the possible role of mitochondria in this process. The present study examined whether BG induced mast cell degranulation, and evaluated the role of calcium transients during mast cell activation. Our investigation focused on the role of the mitochondrial calcium uniporter (MCU) in BG-induced degranulation. Black mouse (C57) bone marrow-derived mast cells were stimulated with 0.5 μg/ml BG, 100 μg/ml peptidoglycan (PGN), or 10 μM A23187 (calcium ionophore), and dynamic changes in cytosolic and mitochondrial calcium and membrane potential were monitored. BG-induced mast cell degranulation occurred in a time-dependent manner, and was significantly reduced under calcium-free conditions. Ruthenium red, a mitochondrial Ca<sup>2+</sup> uniporter blocker, significantly reduced mast cell degranulation induced by BG, PGN, and A23187. These results suggest that the mitochondrial Ca<sup>2+</sup> uniporter has an important regulatory role in BG-induced mast cell degranulation.