The effect of progesterone and 17-β estradiol on membrane-bound HLA-G in adipose derived stem cells

The effect of progesterone and 17-β estradiol on membrane-bound HLA-G in adipose derived stem cells

Membrane-bound HLA-G (mHLA-G) discovery on adipose derived stem cells (ADSCs) as a tolerogenic and immunosuppressive molecule was very important. Many documents have shown that HLA-G expression can be controlled via some hormones such as progesterone (P4) and estradiol (E2). Therefore, this study was designed to evaluate progesterone and estradiol effects on mHLA-G in ADSCs at restricted and combination concentrations. Three independent cell lines were cultured in complete free phenol red DMEM and subcultured to achieve su&#1280; cient cells. These cells were treated with P4, E2 and P4 plus E2 at physiologic and pregnancy concentrations for 3 days in cell culture conditions. The HLA-G positive ADSCs was measured via monoclonal anti HLA-G-FITC/ MEMG-09 by means of flow cytometry in nine groups. Data were analyzed by one way ANOVA and<em> </em>Tukey’s<em> post hoc</em> tests. There were no signi&#768; cant values of the mean percentage of HLA-G positive cells in E2-treated and the combination of P4 plus E2-treated ADSCs compared to control cells (p value&gt;0.05) but P4 had a signi&#768; cant increase on mHLA-G in ADSCs (p value&lt;0.05). High P4 concentration increased mHLA-G but E2 and the combination of P4 plus E₂ could not change mHLA-G on ADSCs.