국산 프로폴리스의 항균활성

As extraction solvent, ethanol fraction was revealed the highest anti-microbial activities. The butanol, ethyl acetate and chloroform fractions were also revealed anti-microbial activities with less extent compared to ethanol fraction. All fractions exhibited to inhibition of bacterial growth regardless gram positive and negative, yeast and fungi, however, was not exhibited effectively to their growth. In the inhibition activities against E. coli, the complete inhibition concentration of Yecheon propolis were at 0.40㎎/ml, respectively. Complete inhibition concentration of Youngwol was revealed at 0.25㎎/ml after 12 hours incubation. In the inhibition activities against S. aureus, the complet inhibition concentration of Yecheon and Youngwol were 0.25 and 0.30㎎/ml, respectively. In the inhibition activities against P. aeruginosa, the complete inhibition concentration of Youngwol propolis was at 0.20㎎/ml. Yecheon propolis was revealed the inhibition at 0.20㎎/ml after 12 hours incubation. In the inhibition activities against S. typhimurium, the complete inhibition concentration of Youngwol propolis was at 0.20 and 0.25㎎/ml and Yecheon was 0.40㎎/ml. The Minimum Inhibitory Concentration (MIC) of ethanol extraction fraction of Yecheon propolis to P. aeruginosa, S. aureus, and S. typhimurium were >0.2, 0.25, 0.35 and 0.4∼>0.5㎎/ml, and of Youngwol propolis to P. aeruginosa, E. coli, S. aureus, S. typhimurium, B. subtilis and C. utilis were 0.15, 0.25, 0.25, 0.25, 0.3 and 0.4㎎/ml, respectively.