α-Lactalbumin 의 암세포 증식 저해효과에 관한 연구

Bovine serum albumin(BSA), α-lactalbumin(α-LA), β-lactoglobulin(β-LG) and bovine immunoglobulin G(IgG) were investigated the cytotoxicity on tumor cell lines, α-LA was showned a tendency of dose dependaent on cytotoxicity using WiDr. The growth of WiDr was inhibited 82% by 1㎎/ml of α-LA. However, IgG, BSA and β-LG were not shown the cytotoxicity on WiDr. When α-LA was purified by using high pressure liquid chromatography(HPLC), the main component(α-LA) was eluted at 33.057 min and extremely small quantities eluted at 32.310 min. The xytotoxicity of main component (eluted at 33.057 min peak) was lower than commercial α-LA. And the cytotoxic activity of hydropyzed α-LA and α-LA treated with EDTA were lower than commercial α-LA on tumor cells.