젖소 초유로부터 TGF-β 1의 정제

Bovine colostrum contains vatious bio-functional proteins. Especially, transforming growth factor-β1 (TGF-β1)has a function in concerns with immune response. The purpose of this study was to establish the purification processing of transforiming growth factor-β1 (TGF-β1). The highest concentration of TGF-β1 was measured within 48 h after parturition in bovine colostrum using ELISA kit. Purification of TGF-β1 from whey protein was carried out by the gel filtration, AF-heparin chromatography and AF-heparin rechromatography. After final purification step, TGF-β1 with a molecular weight of 25 kD was obtained, and confirmed by silver staining and western blotting. Finally, TGF-β1 was identified native form of 25 kD and reducing form of 12.5 kD by reducing agent.