This study was carried out to detect the pro-inflammatory cytokines(IL-1, IL-6, TNF-α, IL-18) and IL-1 receptor in mongolia mare`s milk by western blotting. IL-1 and TNF-α, were detected in 4 samples of mare`s milk. Proteins of 6 kD and 7 kD were bound to specific antibody against hIL-18. But, IL-1 and TNF-α were not detectable in Difco skim milk. IL-6 like factor of 60 kD was detected in both Difco skim milk and mare`s milk. Also, IL-1 receptor accessory of 55 kD was detected in the mongolia mare`s milk.