티벳산 발효유로부터 분리한 Candida kefyr TFP 7의 항균활성 및 항암활성

This study was carried out to investigate antimicrobial and antitumoral activities of Candida kefyr TFP7 isolated from Tibetan fermented milk. Strains of TFP1~10 were isolated from Tibetan fermented milk by agar diffusion method using potato dextrose agar(PDA). Antimicrobial activities were examined against 18 microorganisms of food-related bacteria, yeast, algae, fungi and actinomycetes isolated from soil. Antitumor activities were examined against 9 human tumor cell lines. Strains of TFP2~10 showed strong antimicrobial activities against Micrococcus luteus ATCC 11880, and strains of TFP6~10 to actinomycetes, Streptomyces murinus JCM 4333. In antitumor test, all isolated strains(TFP1~10) showed the growth inhibition of SNU-5 and SW-534 by 60% and 70%, respectively. Among those, the strain TFP7 showed the most antitumor activity, which was 77.5% for SNU-5 and 76.5% for SW-534. The strain was identified as Candida kefye by use of API 20C AUX kit and scanning electron micrograph.