Lactobacillus acidophilus의 장 상피세포에 대한 부착능력 및 장 출혈성 대장균의 부착 억제 능력

The ability of probiotics containing Lactobacillus acidophillus to adhere to the intestinal epithelium may play an important role in colonization of the gastrointestinal tract and preventing enteric pathogen such as enterohemorrhagic E. coli(EHEC) O157:H7. In the study, we investigated the adhesion to human intestinal epithelial cells(Ht-29) of strains of L. acidophilus(3 from human, 2 from pig, and 1 from calf). All of the tested strains of L. acidophilus were highly observed adhesion ability(from 10^(6) to 10^(7) cfu/mL), compared to L.rhamunosus GG as contro. Also, adhered strains of L. acidophilus were significatly preserved in serial wash-out steps. However, no correlation could be observed between cell surface hydrophbicity and adhesion abilities of the tested strains of L. acidophilus. Inhibition of adhesion of EHEC O157:H7 was aksi exanubed, a 2 log cycle reduction was observed by all of the tested strains of L. acidophilus. Theses results suggest that the strains of L. acidophilus with high adhesion ability are resistant to wash-out and adhesion abiltiy inhibition by selected strains of L. acidophilus helps to prevent adhesion of EHEC O157:H7 to intestinal epithelial cells.