담즙산 분해능이 뛰어난 젖산균의 분리 및 동정

The purpose of this study is to isolate probiotic lactic acid bacteria (LAB) that produced bile salts hydrolase. One hundred twenty strains were initially isolated from human feces. Based on their resistance of acid, tolerances of bile salts, and inhibitory activity against Escherichia coli, five strains were selected. A strain producing highest activity of bile salts hydrolase was identified as Lactobacillus plantarum using API carbohydrate fermentation pattern and 16S rRNA sequences, and named CK102. Lactobacillus plantarum CK102 survived at a level of 1.36 × 10^(8)CFU/ml in pH 2 buffer for 6 h and showed exhibited excellent bile tolerance. When L. plantarum CK102 was cultured with E. coil in MRS broth, no viable cells of E. coil was detected after 18 h fermentation. These results suggest that Lactobacillus plantarum CK 102 may be commercially used for the probiotic culture.