젖소 초유 중의 Insulin-like Growth Factor-I 함유 분획이 세포 성장에 미치는 영향

Insulin-like growth factor-I (IGF-Ⅰ) rich fraction, which was obtained molecules ranged between 30kDa and 1kDa, was fractionated by ultrafiltration from bovine colostral whey with 30kDa and 1kDa membrane. IGF-Ⅰ included in fractionated IGF-Ⅰ rich fraction was confirmed by SDS-PAGE and western blotting and then the quantity of IGF-Ⅰ was measured by ELISA. IGF-Ⅰ concentration in IGF-Ⅰ rich fraction was 10ng/mg protein. Effect of IGF-Ⅰ rich fraction on in vitro proliferation of several cells was tested. IEC-6 cell proliferation rate was increased 60%. 53%, 30%, and 20% at 10ng, 1ng, 0.lng, and 0.01ng of IGF-Ⅰ, respectively, compared to control group which was not supplemented by IGF-Ⅰ rich fraction. IGF-Ⅰ rich fraction stimulated in vitro proliferation of IEC-6 cell in a dose dependent manner by increasing cell number. Detroit 551 cell proliferation was enhanced 56% and 26% at 10ng and 1ng level of IGF-Ⅰ, respectively, compared to control group. EL-4 cell and L6 cell proliferation was increased 53% and 46% at 10ng of IGF-Ⅰ, respectively, compared to control group.