HPLC-ELSD를 이용한 발효유 제품 중의 Isomaltooligosaccharides 분석법 개발

A rapid and simple analytical method for the determination of 9 isomaltooligosaccharides (IMO) species in yoghurts was developed using dispersive solid phase extraction (dSPE) clean-up technic and high performance liquid chromatography with evaporative light-scattering detector (HPLC-ELSD). In this study, 9 IMO were extracted from samples simply with chemical reagent using ISO22662 IDF198 method and additional dSPE clean-up. The optimum instrument conditions for the determination were used carbohydrate ES 5μ column with gradient elution of water and acetonitrile and ELS detector. The linearity of this method was expressed as the correlation coefficient (r2), the results of IMO 9 species were shown in 0.9999. LOD and LOQ were respectively 7.9-22.1 mg/kg, 25.9-72.8 mg/kg. The accuracy of intra- and inter day measurements were in the range from 84.3±4.5 to 104.9±6.5%, and the preceision of the intra- and inter-day measurements were in the range from 0.8 to 7.7%. The recoveries were from 84.3±4.5 to 104.9±6.5%. The determination results of IMO 9 species for the 9 yoghurts circulated in the market were in the range from 0.317±0.007 to 1.624±0.050 g/100 g. The newly developed method is appropriate for the determination of IMO in yoghurts, is a rapid and simple method with excellent resolution in compared with previous method.