Isolation of cholesterol-lowering lactic acid bacteria from human intestine for probiotic use
Isolation of cholesterol-lowering lactic acid bacteria from human intestine for probiotic use
- Hyeong-Jun Lim, So-Young Kim, Wan-Kyu Lee
- 대한수의학회
- Journal of Veterinary Science
- 제5권 제4호
- 등재여부 : KCI등재
- 2004.12
- 391 - 395 (5 pages)
Cholesterol-lowering effect of lactic acid bacteria (LAB: Streptococcus, Lactobacillus and Bifidobacterium) is well-known. Thus, we investigated LAB isolated from human intestine on the cholesterol-lowering effect in vitro. Seven Streptococcus (61.1%), 11 Lactobacillus (71.8%) and 7 Bifidobacterium (27.9%) were isolated as acid (pH 2.5 and 3.0) and bile (0.3% oxgall) tolerant strains. Streptococcus HJS-1, Lactobacillus HJL-37 and Bifidobacterium HJB-4 were finally selected as probiotic strains to use through the bile salt hydrolase (BSH) activity assay by using MRS media added taurodeoxycholic acid (TDCA) and the cholesterol-lowering test by using soluble cholesterol containing MRS broth. These studies suggested that the isolated LAB had an excellent hypocholesterolemic effect.