Packaging of Rift Valley fever virus pseudoviruses and establishment of a neutralization assay method
Packaging of Rift Valley fever virus pseudoviruses and establishment of a neutralization assay method
- 대한수의학회
- Journal of Veterinary Science
- 제19권 제2호
-
2018.03200 - 206 (7 pages)
- 이용수 0
초록
Rift Valley fever (RVF) is an acute, febrile zoonotic disease that is caused by the RVF virus (RVFV). RVF is mainly prevalent on the Arabian Peninsula, the African continent, and several islands in the Indian Ocean near southeast Africa. RVFV has been classified by the World Organisation for Animal Health (OIE) as a category A pathogen. To avoid biological safety concerns associated with use of the pathogen in RVFV neutralization assays, the present study investigated and established an RVFV pseudovirus-based neutralization assay. This study used the human immunodeficiency virus (HIV) lentiviral packaging system and RVFV structural proteins to successfully construct RVFV pseudoviruses. Electron microscopy observation and western blotting indicated that the size, structure, and shape of the packaged pseudoviruses were notably similar to those of HIV lentiviral vectors. Infection inhibition assay results showed that an antibody against RVFV inhibited the infective ability of the RVFV pseudoviruses, and an antibody neutralization assay for RVFV detection was then established. This study has successfully established a neutralization assay based on RVFV pseudoviruses and demonstrated that this method can be used to effectively evaluate antibody neutralization.
목차
등록된 목차 정보가 없습니다.
참고문헌 (0)
등록된 참고문헌 정보가 없습니다.
해당 권호 수록 논문 (19)
- Comparative aspects of microRNA expression in canine and human cancers
- Acknowledgement to Reviewers - 2017
- Rapid visual detection of Mycobacterium avium subsp. paratuberculosis by recombinase polymerase amplification combined with a lateral flow dipstick
- Detection of hepatitis E virus genotypes 3 and 4 in pig farms in Korea
- In silico analysis of putative drug and vaccine targets of the metabolic pathways of Actinobacillus pleuropneumoniae using a subtractive/comparative genomics approach
- Pathologic and molecular characterization of Streptococcus dysgalactiae subsp. equisimilis infection in neonatal piglets
- Control of type O foot-and-mouth disease by vaccination in Korea, 2014-2015
- Pathogenic and phylogenetic characteristics of non-O157 Shiga toxin-producing Escherichia coli isolates from retail meats in South Korea
- In vitro treatment of lipopolysaccharide increases invasion of Pasteurella multocida serotype B:2 into bovine aortic endothelial cells
- Korean red ginseng excitation of paraventricular nucleus neurons via non-N-methyl-D-aspartate glutamate receptor activation in mice
- Packaging of Rift Valley fever virus pseudoviruses and establishment of a neutralization assay method
- Calcium-sensing receptor-mediated L-tryptophan-induced secretion of cholecystokinin and glucose-dependent insulinotropic peptide in swine duodenum
- Recombinant-attenuated Salmonella Pullorum strain expressing the hemagglutinin-neuraminidase protein of Newcastle disease virus (NDV) protects chickens against NDV and Salmonella Pullorum challenge
- Cytological endometritis in dairy cows: diagnostic threshold, risk factors, and impact on reproductive performance
- Novel vertebral computed tomography indices in normal and spinal disorder dogs
- Optimization of scan delay for multi-phase computed tomography by using bolus tracking in normal canine kidney
- A two-component signal transduction system contributes to the virulence of Riemerella anatipestifer
- Haemoproteus in barn and collared scops owls from Thailand
- Analysis of protein expression in Brucella abortus mutants with different growth rates by two-dimensional gel electrophoresis and LC-MS/MS peptide analysis