BAPN 으로 유도한 구개열 백서에서 TGF-ß 발현 양상에 대한 연구

A study of TGF-ß Expression Patterns In Cleft Palate Formed Rats Induced by BAPN

Cleft palate has been studied with epidemiologic and molecular methods, and many etiologic factors have been examined closely. Among the research methods, biologic molecule research has been the most important method for cleft palate formation study. The TGF-,8 played an important role in cell migration, epithelial-mesenchymal transdifferentiation, extracellular matrix synthesis and deposition. But there was not much research on the correlation cleft palate induced by beta-aminonitroproprionitrile(BAPN) and TGF-Q expression. The purpose of the present study was to examine how TGF- 9 is expressed in cleft palate rats. 4 Timed-pregnant Sprague-Dawley rats were obtained on the 10th gestation day. On the 13th day of gestation, BAPN-monofurnarate salts ((C3HSN2)z - C4H404) were individually, orally administered to 3 pregnant rats at a ratio of 1g/kg body weight. And 4 pregnant rats were sacrificed on day 20 post coitus (p.c.). The TGF->4 expression in the cleft formed rats fetuses showed the following patterns 1. Osteoblast and mesenchymal cells of the cleft palate rats were of low expression compared with those of the control rats. k 2. The cleft palate rats didn´t show any difference in the TGF- Q expression of osteocyte from the control rats. 3. In western blot analysis, the thickness of band of TGF-1 in the cleft palate rats was thinner and more diluted than that of the control rats.