백서의 실험적 치아이동시 osteonectin 및 osteocalcin의 발현

THE EXPRESSION OF OSTEONECTIN AND OSTEOCALCIN IN THE EXPERIMENTAL TOOTH MOVEMENT IN RAT

백서 전치에 견인력을 가해 비교원단백인 osteonectin 및 osteocalcin의 발현 정도 및 분포 변화를 면역 화학적 염색을 통하여 다음과 같은 결과를 얻었다. 1. 견인력을 가한 후 28일째까지 견인측의 치주인대 섬유는 신장되어 있었고, 압박측의 치주인대 섬유는 압축되어 있었으며 섬유의 배열이 완전히 회복되지 않았다. 2. 대조군의 상아질과 백악질, 골세포에서의 osteonectin 발현은 경미하였지만 조상아세포, 치조골 기질에서는 약양성의 발현을 보였다. 3. 대조군의 상아질, 치수, 치주인대 및 치조골 기질에서의 osteocalcin의 발현은 경미하였지만 치은, 골세포 및 백악질에서는 음성으로 관찰되었다. 4. 실험군의 상아질, 백악질, 치수, 조상아세포에서의 osteonectin 및 osteocalcin의 발현은 견인력 적용 기간에 관계없이 대조군과 큰차이가 없었다. 5. 실험군의 악간보압에서 osteocalcin의 발현은 치아동 7일째까지 대조군과 차이 없다가 14일째 이후에 약양성으로 유지되었으나, osteonectin은 7일째까지 증가되어 강양성을 보이다가 14일째부터 감소되었다. 6. 압박측 치주인대에서의 osteonectin의 발현은 대조군과 차이가 없이 경미한 양상을 나타냈으나 견인측에서는 14일째까지 시간 경과에 따라 증가되었으나 28일째에는 감소되었다. 7. 치주인대에서의 osteocalcin의 발현은 14일째까지 경미했으나 28일째에 견인측이 압밥측보다 많은 강양성을 나타냈고 치아측 보다는 치조골측의 섬유가 더 강한 염색도를 보였다. 8. 치조골 기질에서의 osteocalcin의 발현은 14일째까지 경미한 반응을 나타내다가 28일째에 중등도의 양성 발현을 보였으나 osteonectin의 발현은 4일째까지 대조군과 차이가 없다가 7일째부터 시간에 따라 증가하였으며 압박측보다 견인측에서 발현의 정도가 많았다.

This study was designed to evaluate the expression of non-collagenous protein in periodontal tissue during the experimental movement of rat incisors, by LSAB(labelled streptavidine biotin) immunohistochemical staining for osteonectin and osteocalcin. Twenty seven Sprague-Dawley rats were divided into a control group(3 rats) and 6 experimental groups(24 rats) where 75g of force was applied from helical springs across the maxillary incisors. Rats of experimental groups were sacrificed at 12 hours, 1, 4, 7, 14 and 28 days after force application, respectively. And the tissues of a control group and experimental groups were studied immunohistochemically and histologically. The results were as follows : 1. Until 28 days after force application, periodontal fibers had been strectched on tension side and compressed in pressure side of all the experimental groups, and the arrangement of periodontal fibers had not been recovered yet. 2. The expression of osteonectin in control group was rare in dentin, cementum and osteocyte, and was mild in odontoblasts and matrix of alveolar bone. 3. The expression of osteocalcin in control group was negative in gingiva, osteoblasts, osteocyte and cementum, and was rare in predentin, capillaries in pulp and periodontal ligament and the matrix of alveolar bone. 4. There was no difference in the expression of osteocalcin or osteonectin in dentin, cementum, pulp, odontoblasts, between of control and of experimental groups. 5. The expression of osteonectin in intermaxillary suture got the peak in 7-day and was declined after 14-day. The expression of osteocalcin remained in a same degree since it became mild in 14-day. 6. The expression of osteonectin in pressure side of periodontal ligament of experimental group was rare, which was similar to control group. But in tension side, it wr , increased until 14-day aftrer which it was declined. 7. The expression of osteocalcin in periodntal ligament was rare in 12-hour to 14-day, but became severe in 28-day, which was greater in tension side than in pressure side, and in the periodontal. fiber next to alveolar bone than tc tooth surface. 8. The expression of osteocalcin in alveolar bone was rare until 14-day in pressure side, but became moderate in 28-day. The expression of osteonectin was increased from 7-day by time dependency, which was greater in tension side than in pressure side.