
Isolation and Characterization of an Anti-listerial Bacteriocin from Leuconostoc lactis SD501
Isolation and Characterization of an Anti-listerial Bacteriocin from Leuconostoc lactis SD501
- In-Chan Hwang Ju Kyoung Oh Sang Hoon Kim Se-jong Oh Dae-Kyung Kang
- 한국축산식품학회
- Food Science of Animal Resources
- Korean Journal for Food Science of Animal Resources 제38권 제5호
- 등재여부 : KCI등재
- 2018.10
- 1008 - 1018 (11 pages)
Although bacteriocins with anti-listerial activity have been isolated from a wide variety of lactic acid bacteria, little is known about those from Leuconostoc lactis, a heterofermentative bacterium that produces diacetyl and exopolysaccharides in dairy foods. In this study, an anti-listerial bacteriocin was isolated from Leuc. lactis SD501 and characterized. It was particularly potent against Listeria monocytogenes and also inhibited Enterococcus faecalis. Anti-listerial activity reached a maximum during the early stationary phase and then decreased gradually. The anti-listerial substance was sensitive to proteinase K and α-chymotrypsin, confirming its proteinaceous nature. Its activity remained stable at pH values ranging from 1 to 10. In addition, it was strongly resistant to high temperatures, retaining its activity even after incubation for 15 min at 121℃. The apparent molecular mass of the partially purified anti-listerial bacteriocin was approximately 7 kDa. The characteristics of the SD501 bacteriocin, including its small molecular size (<10 kDa), strong anti-listerial activity, wide pH stability and good thermostability, indicate its classification as a Class Ⅱa bacteriocin.
Introduction
Materials and Methods
Results and Discussion
Conclusion