BALB/c Mouse 모델에서 Photofrin을 이용한 광역학 치료의 효과

A Study of Photodynamic Therapy in a BALB/c Mouse Model

Photodynamic therapy is a non-invasive cancer therapy that has recently attracted much attention in the field of cancer treatment. This study is an experiment to observe the effect of laser dose difference in photodynamic therapy. Murine breast cancer cells, EMT6 cells, were used to generate tumor bearing mouse models. The experimental group was divided into 5 groups as control group, laser 100 J/cm², 200 J/cm², 300 J/cm² and 400 J/cm² irradiation group. Photofrin was administered intraperitoneally and after 48 hours, laser irradiated on tumor with energy 0-400 J/cm². the tumor volume was measured with a digital caliper on day 0, day 2, day 4, day 8 and day 12. The volume of tumor after photodynamic therapy was increased in the control group, laser 100 J/cm² irradiation group and laser 200 J/cm² irradiation group until day 12 after irradiation. Both the laser 300 J/cm² irradiated group and the laser 400 J/cm² irradiated group showed a decrease in the tumor volume from 2 to 12 days after irradiation. These results suggested that treatment effect will be obtained at a dose of 300 J/cm² or more in the experiment using the tumor bearing mouse model and photoprin.