
하이드로젤 지지체 기반 3차원 환경에서 개 간엽줄기세포의 분화능 분석
Differentiation potential of canine mesenchymal stem cells on hydrogel scaffold-based three-dimensional environment
- 구나연(Na-Yeon Gu) 박미정(Mi Jeong Park) 이지현(Jienny Lee) 변정수(Jeong Su Byeon) 정다운(Da-Un Jeong) 조인수(In-Soo Cho) 차상호(Sang-Ho Cha)
- 대한수의학회
- Korean Journal of Veterinary Research(구 대한수의학회지)
- 제58권 제4호
- 등재여부 : KCI등재
- 2018.12
- 211 - 217 (7 pages)
Mesenchymal stem cells (MSCs) are useful candidates for tissue engineering and cell therapy. Physiological cell environment not only connects cells to each other, but also connects cells to the extracellular matrix that provide mechanical support, thus exposing the entire cell surface and activating signaling pathways. Hydrogel is a polymeric material that swells in water and maintains a distinct 3-dimensional (3D) network structure by cross linking. In this study, we investigated the optimized cellular function for canine adipose tissue-derived MSCs (cAD-MSCs) using hydrogel. We observed that the expression levels of Ki67 and proliferating cell nuclear antigen, which are involved in cell proliferation and stemness, were increased in transwell-hydrogel (3D-TN) compared to the transwell-normal (TN). Also, transforming growth factor-β1 and SOX9, which are typical bone morphogenesis-inducing factors, were increased in 3D-TN compared to the TN. Collagen type II alpha 1, which is a chondrocyte-specific marker, was increased in 3D-TN compared to the TN. Osteocalcin, which is a osteocyte-specific marker, was increased in 3DTN compared to the TN. Collectively, preconditioning cAD-MSCs via 3D culture systems can enhance inherent secretory properties that may improve the potency and efficacy of MSCs-based therapies for bone regeneration process.
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