층층둥굴레(Polygonatum stenophyllum Maxim.)의 캘러스 유도 및 기내 식물체 분화

Callus induction and in vitro plant regeneration of Polygonatum stenophyllum Maxim.

APolygonatum stenophyllum Maxim. is an important endangered plant belonging to the family Liliaceae. A method was developed for the rapid micropropagation of P. stenophyllum through plant regeneration from rhizome (1-year, 3-years, and 5-years) explant-derived calli. The rhizome segments were cultured in Murashige and Skoog (MS) medium supplemented with varying concentrations of 2,4-D (0, 0.5, 1.0, 1.5 mg·L-1) for callus induction. In media supplemented with 0.5 mg·L-1 of 2,4-D, 87% of 3-years rhizome produced callus. Subsequently, the callus was transferred to 1/2MS medium supplemented with various concentrations of IAA, IBA, NAA, and 2,4-D (0, 0.1, 0.5 and 1.0 mg·L-1) for adventitious shoot formation. The highest percentage of adventitious shoot induction (57%) was observed in 1/2MS medium containing 0.5 mg·L-1 of NAA. Elongation of the adventitious shoot was achieved in 1/2MS medium supplemented with 0.1 mg·L-1 of BA. Rooting was achieved in 1/2MS medium without any hormones. It is hypothesized that the stated in vitro propagation protocol will be useful for conservation and mass propagation of the endangered Polygonatum stenophyllum Maxim. for bio-resources.