배 ‘원황’의 재분화와 발근에 미치는 식물생장조절물질과 탄소원의 영향

Effect of plant growth regulators and carbon source on the shoot regeneration and rooting of ‘Wonhwang’ pear (Pyrus pyrifolia L.)

The aim of this research is to establish shoot regeneration system for `Wonhwang` pear (Pyrus pyrifolia L.) using various concentrations of 1-Naphthaleneacetic acid (NAA) 0.01, 0.05, 0.1, 0.5 mg/L in combination with benzylaminopurine (BA) 3, 5, 10 mg/L. Medium containing 4.4 g/L of Murashige and Skoog (MS) medium with vitamins containing 8 g/L of plant agar and 30 g/L of sucrose with NAA 0.05 mg/L and BA 3 mg/L showed 13.3% of shoot regeneration rate. `Wonhwang` showed no root growth on existing rooting media of P. pyrifolia cv. Niitaka, `Whangkeumbae` and `Bae Yun No. 3`. We evaluated the effect of concentration and kinds of plant growth regulators and carbon source to establish efficient rooting condition for `Wonhwang` pear. In the result of using various concentrations of NAA 0.5 mg/L and 1.0 mg/L in combination with indolebutyric acid (IBA) 3, 5, 10 mg/L, rooting rate of 24% was observed using 1/4 Linsmaier and Skoog (LS) medium supplemented with 7.5 g/L glucose as carbon source and IBA 1.0 mg/L with NAA 1.0 mg/L.