멸종위기종인 솔나리(Lilium cernum Komarvo.)의 인편 유래 캘러스 유도 및 기내 식물체 재분화
Plant regeneration through the callus culture induced from bulb scales of an endangered species Lilium cernum Komarvo.
- 배기화(Kee Hwa Bae) 윤의수(Eui Soo Yoon)
- Journal of Plant Biotechnology
- 40권 2호
- 등재여부 : KCI등재
- 65 - 71 (7 pages)
Lilium cernum Komarvo. is an important endangered plant belonging to the family Liliaceae. A method was developed for the rapid micropropagation of L. cernum through plant regeneration from bulb scales explant-derived calli. The bulb scales segments were cultured on Murashige and Skoog (MS) medium supplemented with 0, 0.5, 1.0, 3.0 mg·L-1 kinetin and 0, 0.1, 0.5, 1.0 mg·L-1 NAA or 2,4-D for callus induction. In media with 0.5∼3.0 mg·L-1 kinetin and 0.1∼1.0 mg·L-1 NAA and 2,4-D, 95∼100% of explants produced callus. They were then transferred to MS medium supplemented with various concentrations of NAA (0, 0.01, 0.05 and 1.0 mg·L-1) in combination with BA (0, 1.0 and 2.0 mg·L-1) for bulbet formation. Bulbet induction (78%), weight (468 mg) and size (15.5 mm) were obtained the highest on MS medium containing 2.0 mg·L-1 BA and 1.0 m g·L-1 NAA. In vitro frequency of plant regeneration was not significantly treated in strength of MS and sucrose concentration. Chlorophyll contents in 1/2MS with 50 g·L-1 sucrose trea ments were higher than those in control and another treatment. This in vitro propagation protocol will be useful for conservation and mass propagation of this endangered plant.
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