희귀수종인 주걱비름(Sedum tosaense Makino)의 잎절편으로부터 기내 식물체 재분화 및 광독립배양을 통한 토양순화

Plant regeneration and soil acclimatization through photoautotrophic culture from leaf explant of a rare species in Sedum tosaense Makino

The aim of this study was to establish plant regeneration from leaf explants of Sedum tosaense Makino, which is globally rare and endangered species. The leaf explants of S. tosaense were cultured on the MS medium supplemented with different concentration of BA and NAA for callus induction. Callus induction was showed the highest (100%) on MS medium containing 2.0 mg·L-1 BA and 1.0 m g·L-1 NAA. The highest number of shoots were regenerated when callus were cultured on MS medium containing 2.0 m g·L-1 BA and 1.0 mg·L-1 NAA for 5 weeks. The axillary bud were cultured on the MS media supplemented with combination of BA and NAA for in vitro propagation. The highest number of adventitious shoot (7.9 per explants) formed at 1.0 mg·L-1 NAA and 2.0 mg·L-1 BA. For rooting, MS medium supplemented with or without 2.0 g·L-1 activated charcoal was tested. The optimal results were observed using MS medium supplemented with 2.0 g·L-1 activated charcoal, on which 85.7 (No. of root), 4.6 cm (length of root). 1,200 ppm CO2 and 50 ppm CO2 were supplied for make certain the effects of CO2 on pre-acclimatization by photoautotrophic culture. 1,200 ppm CO2 treatment was established higher than 350 ppm CO2 treatment. Soil acclimatization of in vitro plantlets was the best in mixture soil consisted of peat moss and perlite with 100% survival rate and they showed the maximum growth.