Enhanced bacterial resistance in transgenic tobacco expressing a BrRZFP1 ending a C3HC4-type RING zinc ginger protein from Brassica rapa

C3H4-type RING zinc finger protein (BrRZP1) from Brassica rapa under abiotic stresses. To elucidate the role of the BrRFP1 transcription factor in gene regulation, we transformed tobacco plants with the BrRZFP1 gone. Plants were regenerated from 82 independently transformed callus lines of tobacco and analysed for transgene expression. Trans-gene integration and expression was confirmed by Southern and RT-PCR analyses, respectively. T2 plants displayed more tolerance to the bacterial pathogens Pectobacterium carotovorum and Ralstonia solanacearum, and the tolerance levels were correlated with BrRZFP1 expression levels. These results suggest that the transcription factor BrRZFP1 is an important determinant of stress response in plants and its overexpression in plants could increase biotic stress resistance.