FT 유전자 형질전환 스프레이 국화 (Dendranthema grandiflorum (Ramat.) Kitamura)의 조기개화성

FT-transgenic spray-type Chrysanthemum (Dendranthema grandiflorum Kitamura) showing early-flowering

The flowering locus T (FT) gene, of which expression will be controlled at high temperature by heat shock promoter (it printed as to HSproFT), was introduced into spray-type chrysanthemum (Dendranthema grandiflorum(Ramat.) Kitamura) 2 cultivars (``Pink PangPang`` and ``Pink Pride`` by co-cultivation with Agrobacterium tumefaciens strain C58C1 harboring pCAMBIA2300 containing the HSproFT gene. After leaf segments of the 2 cultivars were infected with the A. tumafaciens with C58C1 as explants, shoots were regenerated from the explants cultured on the 1st selection medium (MS basal salts+1.0 mg/L BA, 0.5 mg/L IAA+10 mg/L kanamycin+0.7% plant agar, pH 5.8). The shoots were transferred into the 2nd selection medium (MS basal salts+1.0 mg/L BA, 0.5 mg/L IAA+20 mg/L kanamycin+0.7% plant agar, pH 5.8). One hundred seventeen plantlets from ``Pink PangPang`` and 5 ones from ``Pink Pride`` were confirmed as transformants by PCR analysis. Twenty six of the transformants and non-transformants were acclimatized and established well in a green house. Eights of 26 transgenic lines showed flower bud 1.7~10 days earlier than nontransgenic plants, and 24 of them flowered 1~6 days earlier than non- transgenic plants. The shape and color of flower of all HSproFT-transgenic lines were not different with those of non- transgenic plants.