Bioreactor를 이용한 사계성 딸기 조직배양묘 대량증식을 위한 적정 무호르몬 배지

Suitable hormone-free medium for in vitro mass propagation via bioreactor culture of ever-bearing strawberry

This study was carried out to determine optimal medium conditions for mass propagation by bioreactor culture of ever-bearing strawberry (Fragaria × ananassa D.). Two different type of nitride were that mixed NH4NO3 and KNO3 or added KNO3 only. And nitride concentrations were at the 4 levels of 1/2×, 1×, 2× and 3× that was included NH4NO3 and KNO3. Sucrose content ranged at 3 levels of 10 g·L -1 , 20 g·L -1 and 30 g·L -1 and medium pH were at the 3 levels of 4.6, 5.6 and 6.6. In bioreactor culture, medium that are included NH4NO3 and KNO3 together in MS medium was suitable for mass propagation. Medium EC rose rapidly when the nitride concentration was increased. For that reason, plantlet growth was inhibited. Shoots of nitride 1/2× concentration was 10.8 ea and 1/2× concentration was suitable for shoot propagation. Fresh weight of sucrose 30 g·L -1 was 3,101 mg which was heaviest and aerial and ground part were higher than the other concentration. Shoots were increased in proportion to the increasing concentration of sucrose. In the pH condition, from pH 5.6 to 6.8 were appropriate for the optimum growth of aerial and ground part of plant. From the results, in bioreactor culture for mass propagation, MS medium was suitable 1/2×concentration that was included NH4 NO3 and KNO3 together, and added 30 g·L-1 of sucrose, and then adjusted pH between 5.6 and 6.6.