Imaging and analysis of genetically encoded calcium indicators linking neural circuits and behaviors
- 대한생리학회-대한약리학회
- The Korean Journal of Physiology & Pharmacology
- 제23권 제4호
- : SCOPUS, SCIE, KCI등재
- 2019.07
- 237 - 249 (13 pages)
Confirming the direct link between neural circuit activity and animal behavior has been a principal aim of neuroscience. The genetically encoded calcium indicator (GECI), which binds to calcium ions and emits fluorescence visualizing intracellular calcium concentration, enables detection of in vivo neuronal firing activity. Various GECIs have been developed and can be chosen for diverse purposes. These GECI-based signals can be acquired by several tools including two-photon microscopy and microendoscopy for precise or wide imaging at cellular to synaptic levels. In addition, the images from GECI signals can be analyzed with open source codes including constrained non-negative matrix factorization for endoscopy data (CNMF_E) and miniscope 1-photon-based calcium imaging signal extraction pipeline (MIN1PIPE), and considering parameters of the imaged brain regions (e.g., diameter or shape of soma or the resolution of recorded images), the real-time activity of each cell can be acquired and linked with animal behaviors. As a result, GECI signal analysis can be a powerful tool for revealing the functions of neuronal circuits related to specific behaviors.
INTRODUCTION
WHY GECI?
CALCIUM DYNAMICS
GECI DEVELOPMENT
IN VIVO GECI IMAGING METHODS
ANALYSIS OF GECI IMAGES
CONCLUSION