노화흰쥐의 해마에서 parvalbum in과 calbindin-D 28K의 면역반응세포에 관한 연구

Studies on the Parvalbumin and Calbindin-D28K immunoreactive Neurons in the Hippocampus of Aged Rats

Aging of the hippocampus has been characterized by generative changes of fiber connetions, cell loss, and involved memory and learning impariment, cognition deficit. Aging leads to alternation in activity of parvalbumin (PV) and calbindin-D28k(CB) expressing in the brain of the rats. Two of calcium binding proteins (CBPs), PV and CB are, widely expressed in the adult central nuervous system. The aim of the present study is, therefore, to investigative possible changes in the number and morphology of PV and CB-immunoreactive neurons with aging in each area of the hippocampus of aged rats. The number and density of PV-immunoreactive neurons in the dendate gyrus and CA1 subregion was decreased in the hippocampus in aged group when compared with the young group. But, the number of PV-immunoreactive neurons in the CA3 subregion of the aged group was similar to that in the young group. And, excepting the CA1 subregion, the number of CB-immunoreactive neurons was not practically different in the young group when compared with the aged group. These results showed the alterative expression of the two CBPs during aging process of hippocampus. The age-related reduction of CBPs in hippocampus subregion is likely to deprive neurons from the capacity to better intracellular calcium and to leave them vulnerable to pathological processes.