HL-60세포에서 disulfiram의 항암작용과 미토콘드리아 안정성에 대한 연구

Anti-tumor activity and mitochondrial stability of disulfiram in HL-60 cells

Disulfiram (DSF) is a member of the dithiocarbamate family that can bind copper. Recent studies have shown that DSF hasanti-cancer activities, but the mechanism has not been clarified. Therefore, it is important to study the action mechanism of DSF tomaximize its anticancer effects. A human leukemia cell line, HL-60, was used in this study. HL-60 cells were treated with DSF and thecellular metabolic activity was measured. DSF increased the cell death of HL-60 cells in annexin V-fluorescein isothiocyanate/propidium iodide staining analysis. In addition, DSF decreased the mitochondrial membrane potential (MMP) of the HL-60 cells. Thecytotoxicity of DSF on HL-60 cells was observed at 0.4 μM. Interestingly, the reduction of MMP by DSF was recovered by N-acetyl-L-cysteine, an inhibitor of reactive oxygen species (ROS) production. This suggests that the decrease in MMP by DSF is closelyrelated to the production of ROS in HL-60 cells, which indicates the relationship between the apoptosis of HL-60 cells by DSF and therole of the mitochondria. This study provides clinicians and researchers with valuable information regarding the anti-cancer activity ofDSF in terms of the action mechanism.