
A Novel Multiplex-PCR Assay to Detect Three Non-Halal Meats Contained in Meatball using Mitochondrial 12S rRNA Gene
- Muhammad Cahyadi Tommy Wibowo Ahmad Pramono Zakaria Husein Abdurrahman
- 한국축산식품학회
- Food Science of Animal Resources
- Food Science of Animal Resources 제40권 제4호
- 등재여부 : KCI등재
- 2020.07
- 628 - 635 (8 pages)
The objective of this study was to detect three non-halal meat products consisted of dog, pork, and rat species in meatball using novel multiplex-PCR with 12S rRNA gene as target sites. A total of 33 self-made meatballs were used, and they were grouped into eleven types of meatball based on meat species origin contained in the meatballs. Each type consisted of three meatballs. Extraction of genomic DNA from the meatballs was used as a DNA template for simplex-, duplex-, and multiplex-PCR processes. The result of simplex-PCR, duplex-PCR, and multiplex-PCR showed that the 12S rRNA primer gene successfully amplified DNA for each species bovine, dog, pig, and rat, which are respectively indicated by 155, 244, 357, and 491 bp of DNA bands. In addition, multiplex-PCR with 12S rRNA gene primers can be uniquely and accurately used for detection bovine, dog, pig, and rat species on beef meatball in one reaction.
Introduction
Materials and Methods
Results
Discussion
Conflicts of Interest
Acknowledgements
Author Contributions
Ethics Approval
References