Cnidium officinale, Fungal disease, Fusarium solani, Fusarium wilt

The aim of the present study was to develop a sensitive and specific detection method for the rapid detec- tion of apple scar skin viroid (ASSVd) in apple leaves. The resulting reverse transcription recombinase poly- merase amplification (RT-RPA) assay can be completed in 10 min at 42oC, is 10 times more sensitive than con- ventional reverse transcription polymerase chain reaction, and can specifically amplify ASSVd without any cross-reactivity with other common apple viruses, including apple stem grooving virus, apple stem pitting virus, and apple chlorotic leaf spot virus. The reliability of the RT-RPA assay was assessed, and the findings suggested that it can be successfully utilized to detect ASSVd in field-collected samples. The RT-RPA assay developed in the present study provides a potentially valuable means for improving the detection of ASSVd in viroid-free certification programs, especially in resource-limited conditions.