
Next-generation sequencing for the genetic characterization of Maedi/Visna virus isolated from the northwest of China
- Ling Zhao Liang Zhang Xiaona Shi Xujie Duan Huiping Li Shuying Liu
- 대한수의학회
- Journal of Veterinary Science
- 제22권 제6호
- 등재여부 : KCI등재
- 2021.11
- 1 - 9 (9 pages)
Background: Maedi/Visna virus (MVV) is a contagious viral pathogen that causes considerable economic losses to the sheep industry worldwide. Objectives: In China, MVV has been detected in several regions, but its molecular characteristics and genetic variations were not thoroughly investigated. Methods: Therefore, in this study, we conducted next-generation sequencing on an MVV strain obtained from northwest China to reveal its genetic evolution via phylogenetic analysis. Results: A MVV strain obtained from Inner Mongolia (NM) of China was identified. Sequence analysis indicated that its whole-genome length is 9193 bp. Homology comparison of nucleotides between the NM strain and reference strains showed that the sequence homology of gag and env were 77.1%-86.8% and 67.7%-75.5%, respectively. Phylogenetic analysis revealed that the NM strain was closely related to the reference strains isolated from America, which belong to the A2 type. Notably, there were 5 amino acid insertions in variable region 4 and a highly variable motif at the C-terminal of the surface glycoprotein (SU5). Conclusions: The present study is the first to show the whole-genome sequence of an MVV obtained from China. The detailed analyses provide essential information for understanding the genetic characteristics of MVV, and the results enrich the MVV library.
INTRODUCTION
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RESULTS
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ACKNOWLEDGEMENTS
SUPPLEMENTARY MATERIALS
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