Anti-cancer effects of fenbendazole on 5-fluorouracil-resistant colorectal cancer cells
Anti-cancer effects of fenbendazole on 5-fluorouracil-resistant colorectal cancer cells
- 대한생리학회-대한약리학회
- The Korean Journal of Physiology & Pharmacology
- 제26권 제5호
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2022.09377 - 387 (11 pages)
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DOI : 10.4196/kjpp.2022.26.5.377
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키워드
초록
Benzimidazole anthelmintic agents have been recently repurposed to overcome cancers resistant to conventional therapies. To evaluate the anti-cancer effects of benzimidazole on resistant cells, various cell death pathways were investigated in 5-fluorouracil-resistant colorectal cancer cells. The viability of wild-type and 5-fluorouracil-resistant SNU-C5 colorectal cancer cells was assayed, followed by Western blotting. Flow cytometry assays for cell death and cell cycle was also performed to analyze the anti-cancer effects of benzimidazole. When compared with albendazole, fenbendazole showed higher susceptibility to 5-fluorouracil-resistant SNU-C5 cells and was used in subsequent experiments. Flow cytometry revealed that fenbendazole significantly induces apoptosis as well as cell cycle arrest at G2/ M phase on both cells. When compared with wild-type SNU-C5 cells, 5-fluorouracilresistant SNU-C5 cells showed reduced autophagy, increased ferroptosis and ferroptosis- augmented apoptosis, and less activation of caspase-8 and p53. These results suggest that fenbendazole may be a potential alternative treatment in 5-fluorouracilresistant cancer cells, and the anticancer activity of fenbendazole does not require p53 in 5-fluorouracil-resistant SNU-C5 cells.
목차
INTRODUCTION
METHODS
RESULTS
DISCUSSION
FUNDING
ACKNOWLEDGEMENTS
CONFLICTS OF INTEREST
REFERENCES
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