Solanum pinnatisectum 엽록체 전장유전체 구명 및 특이적 분자마커의 개발

Completion of chloroplast genome sequence and development of PCR-based markers for Solanum pinnatisectum

Solanum pinnatisectum, originating from Mexico, is a wild potato species valued for its resistance to various insects and pathogens, including the Colorado potato beetle, potato tuber moth, Phytophthora infestans, and Erwinia carotovorum. Despite its advantageous traits, S. pinnatisectum is diploid with an endosperm balanced number (EBN) of one, presenting sexual reproduction barriers with cultivated potatoes. Cell fusion methods can be employed to circumvent these barriers and integrate beneficial traits from S. pinnatisectum into cultivated potatoes. In this study, therefore, the chloroplast genome sequence of S. pinnatisectum was completed using next-generation sequencing (NGS) technology and compared with those of ten other Solanum species to develop specific PCR markers for S. pinnatisectum. The complete chloroplast genome of S. pinnatisectum is 155,597 bp in length and exhibits structural organization similar to those of other Solanum species. Sequence alignment of the complete chloroplast genomes sequences of eleven Solanum species, including S. pinnatisectum, identified 216 SNPs and 26 InDels unique to S. pinnatisectum. Based on these InDels and SNPs, six PCR-based markers were developed to identify the plastid genotype of S. pinnatisectum. The results of this study will be instrumental in distinguishing S. pinnatisectum from other Solanum species, selecting suitable genotypes for potato breeding through somatic hybridization, and accelerating the breeding program using S. pinnatisectum.