엽록체 전장유전체 정보를 이용한 PCR 기반의 S. iopetalum 및 S. mochiquense 특이적 분자마커의 개발

Development of PCR-based markers specific to Solanum iopetalum and S. mochiquense by using complete chloroplast genome sequences of Solanum species

Solanum iopetalum and S. mochiquense originating from Mexico and Peru, respectively are valuable genetic resources for developing new potato cultivars due to their resistance to diverse biotic and abiotic stresses, including drought, late blight, potato leafroll virus, root-knot nematode, potato virus X and potato virus Y. However, there are barriers to sexual reproduction between these wild species and the cultivated potato due to differences in their ploidy levels and Endosperm Balanced Number (EBN) values. Somatic fusion offers a potential approach to overcoming this reproductive barriers. After somatic fusion, it is necessary to select suitable hybrids using molecular markers. In this study, we developed specific markers for each of S. mochiquense and S. iopetalum by comparing their chloroplast genome sequences obtained using next-generation sequencing technology with those of other Solanum species. Comparing the chloroplast genomes of these two wild species with those of other Solanum species revealed their similar structure, length, gene number, and composition. These results were further supported by phylogenetic analysis, which indicated a close relationship among the Solanum species. Sequence alignments of chloroplast genomes identified many specific InDel and SNP regions, and we developed five PCR markers based on these InDels and SNPs for each of S. mochiquense and S. iopetalum. The results of this study will contribute to our understanding of the evolution of Solanum species and to the development of new potato cultivars utilizing these two wild species.