Solanum kurtzianum 엽록체 전장유전체 분석 및 이를 이용한 S. kurtzianum 특이적 PCR 기반의 분자마커 개발

Completion of chloroplast genome sequence of Solanum kurtzianum and development of PCR-based markers specific to S. kurtzianum

Solanum kurtzianum, the wild potato species originating from Argentina, exhibits resistance to abiotic stresses such as drought, heat, and salinity, as well as biotic factors such as blackleg, potato wart, and potato leafroll virus. These traits make it a valuable resource for potato breeding. However, S. kurtzianum and S. tuberosum differ in EBN (Endosperm Balance Number) values (two and four respectively), direct crossing is not feasible. Therefore, somatic cell fusion can be utilized to overcome this limitation. In this study, we report the complete chloroplast genome of S. kurtzianum, assembled using next generation sequencing technology and the development of S. kurtzianum-specific markers based on the comparison of S. kurtzianum cpDNA with those of other Solanum species. The S. kurtzianum cpDNA was 155,522 bp in length, and its structure was nearly identical to those of other Solanum species. Phylogenetic analysis using cpDNAs of S. kurtzianum and 21 other species in the Solanaceae family revealed that S. kurtzianum is most closely related to S. brevicaule. Multiple sequence alignment of the complete cpDNA of S. kurtzianum and 10 other Solanum species identified 26 SNPs specific to S. kurtzianum and one InDel specific to both S. kurtzianum and S. hougasii. Based on these polymorphisms, four SNP- and one Indel-based PCR markers specific to S. kurtzianum were developed. The results of this study will advance understanding of evolutionary dynamics of Solanum species and support the breeding of new potato varieties using S. kurtzianum.